发明名称 |
Method for producing isopropanol and recombinant yeast capable of producing isopropanol |
摘要 |
Isopropanol is produced with good productivity via fermentation processes. Specifically, isopropanol is produced at a high level in a medium by culturing recombinant yeast into which an acetoacetyl CoA synthase gene and a group of genes (isopropanol synthesis-related gene group) encoding a group of enzymes for synthesis of isopropanol from acetoacetyl CoA have been introduced. |
申请公布号 |
US8828693(B2) |
申请公布日期 |
2014.09.09 |
申请号 |
US201013697626 |
申请日期 |
2010.05.14 |
申请人 |
Toyora Jidosha Kabushiki Kaisha |
发明人 |
Muramatsu Masayoshi;Yoneda Satoshi |
分类号 |
C12N15/52 |
主分类号 |
C12N15/52 |
代理机构 |
Sughrue Mion, PLLC |
代理人 |
Sughrue Mion, PLLC |
主权项 |
1. A method for producing isopropanol, comprising culturing recombinant yeast into which a heterologous acetoacetyl CoA synthase gene encoding an enzyme that catalyzes a reaction for conversion of acetyl CoA and malonyl CoA to acetoacetyl CoA and an isopropanol-biosynthesis-related gene group associated with a metabolic pathway for synthesis of isopropanol from acetoacetyl CoA are introduced, wherein the acetoacetyl CoA synthase gene is a gene (ORFn gene) obtained from a microorganism of the genus Streptomyces and the isopropanol-biosynthesis-related gene group comprises an isopropanol dehydrogenase gene (pdh gene) from a Clostridium-beijerinckii, thereby obtaining a recombinant yeast expressing the ORFn gene and the pdh gene, and then obtaining isopropanol from the culture product of the recombinant yeast. |
地址 |
Aichi-ken JP |