MULTI-SPOT METAL-CAPPED NANOSTRUCTURE ARRAY NUCLEIC ACID CHIP FOR DIAGNOSING OF CORNEAL DYSTROPHY AND PREPARATION METHOD THEREOF PRODUCING SAME

摘要

A multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy, and more particularly to a multi-spot metal-capped nanostructure array nucleic acid chip capable of employing LSPR (localized surface plasmon resonance) optical properties, a preparation method thereof, and a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations, which can diagnose various corneal dystrophies. The metal-capped nanostructure array nucleic acid chip can be combined with analysis devices, including a light source, a detector, a spectrophotometer and a computer, to provide an LSPR optical property-based optical biosensor, and the use of the multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations allows the simultaneous diagnosis of various corneal dystrophies that are genetic ocular diseases.

主权项

1. A method of preparing a multi-spot metal-capped nanostructure array nucleic acid chip, the method comprising the steps of:
(a) forming a metal thin film layer (second layer) on a substrate (first layer); (b) forming multi-spots on the metal thin film layer (second layer) and arraying nanostructures on the respective surfaces of the multi-spots at regular intervals to form a nanostructure layer (third layer); (c) forming a metal thin film layer on the nanostructure layer (third layer); and (d) immobilizing probe nucleic acids on the metal thin film layer (fourth layer).