SYSTEM AND METHOD TO SEPARATE CELLS AND/OR PARTICLES

摘要

The disclosure related to a method and system to separate a subgroup of objects from a mixture of objects present in a suspension wherein said suspension if exposed to acoustic forces acting in one or two dimensions, which forces said objects to separate from each other into groups which have similar properties in connection with size and/or mass density and/or compressibility.

主权项

1. A micro scaled method for separating a subgroup of cells and/or particles from a mixture of different types of cells and/or particles in a suspension, comprising the steps of;
i) subjecting a suspension to pressure, wherein said pressure forces said suspension into one inlet and into at least one pre-alignment channel present on a microfluid chip, ii) subjecting said suspension to a two dimensional acoustic force directed perpendicular to the length direction of the pre-alignment channel, iii) forcing said mixture of cells and/or particles and infusing cell free buffer solution through a second inlet simultaneously into at least one separation channel, wherein said mixture of cells and/or particles are forced towards the walls of the channel, iv) subjecting said cells and/or particles to a one dimensional acoustic force directed perpendicular to the length direction of the separation channel and v) collecting cells and/or particles having the same size and/or mass density and/or compressibility from said second channel present on the microfluid chip through at least two outlets.