Methods for identifying candidate modulators of NOTUM activity

摘要

The invention provides compositions and methods of use for identifying modulators of NOTUM, e.g., NOTUM inhibitors. In some aspects, identified compounds are useful for modulating Wnt signaling at sites of tissue damage. The invention further provides methods of promoting regeneration by inhibiting NOTUM.

主权项

1. A method of identifying a candidate modulator of NOTUM activity comprising: (a) providing a composition comprising: (1) an isolated NOTUM polypeptide; (2) a reporter molecule comprising a GPI anchor and a detectable moiety; and (3) a test compound, wherein the reporter molecule is attached to a membrane via the GPI anchor; (b) maintaining the composition for a suitable time period; (c) and measuring the amount of detectable moiety released from the membrane during the time period, wherein release of a greater amount of detectable moiety than would occur in the absence of the test compound indicates that the test compound is a candidate enhancer of NOTUM activity, and release of a lower amount of the detectable moiety as compared with the amount that would occur in the absence of the test compound indicates that the test compound is a candidate inhibitor of NOTUM activity.