HIGH FIDELITY DETECTION OF NUCLEIC ACID DIFFERENCES BY LIGASE DETECTION REACTION

摘要

PROBLEM TO BE SOLVED: To distinguish a minority template in the presence of an excessive normal template.SOLUTION: Ligase detection reaction is utilized to distinguish a minority template in the presence of an excessive normal template with a thermostable ligase. This process can be carried out with a mutant ligase, a thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of the minority template.