| 摘要 |
FIELD: biotechnology.SUBSTANCE: method of production of antirabic vaccine for animals comprises preparing of inoculum from the strain of rabies virus, infection contamination with the inoculum of the culture of the continuous cells, culturing the rabies virus, collection of virus-containing suspension with its subsequent inactivation and preparation of the target product, at that the inactivation is carried out by adding the virus-containing suspension to ethanol in the final concentration of 18-20%, exposure for 20-22 hours at a temperature of 36-37°C under constant stirring, then 4-6% aluminium hydroxide is added in a ratio to the reaction mixture of 1:(4.5-5.0).EFFECT: simplification of the method and improvement of quality of the product by increasing the shelf life of the target product.2 ex |
