HO/LL canola with resistance to clubroot disease

摘要

This disclosure concerns a plant of the genus, Brassica, or parts thereof, which comprise one or more traits selected from the group consisting of high oleic acid content, low linolenic acid content, increased herbicide resistance, restorer of cytoplasmic male sterility, and increased clubroot disease (Plasmodiophora brassicae) resistance, compared to a wild-type plant of the same species. This disclosure further relates to wild-type and mutant alleles of genes involved in these traits, molecular markers linked thereto, and methods of their use.

主权项

1. A method for selecting a Brassica napus plant comprising a DNA sequence contributing to clubroot disease resistance in Brassica napus, the method comprising:
isolating genomic DNA from a B. napus plant; and screening the isolated genomic DNA for a nucleic acid molecular short sequence repeat (SSR) marker comprising a polynucleotide that is amplified in a polymerase chain reaction by the primers of SEQ ID NO:16 and SEQ ID NO:17 and is 300 nucleotides in length; selecting a plant comprising a DNA sequence contributing to clubroot disease resistance in Brassica napus if the plant comprises the SSR marker; and cultivating the selected plant.