发明名称 |
FLUORESCENT DETECTION OF IN VITRO TRANSLATED PROTEIN ON A SOLID SURFACE |
摘要 |
Disclosed herein are methods and kits useful in the detection of protein folding and in the identification of compounds that promote proper protein folding. In one example approach, fluorophores and a protein tag are incorporated into a nascent polypeptide within a ribosome-nascent-chain complex during cell free translation and the resulting labeled ribosome-nascent-chain complex is conjugated to a solid surface via the tag. Fluorescence imaging via FRET is then preformed to assess the folding state of the ribosome-nascent-chain complex under a variety of conditions. |
申请公布号 |
US2014099724(A1) |
申请公布日期 |
2014.04.10 |
申请号 |
US201314050177 |
申请日期 |
2013.10.09 |
申请人 |
SKACH WILLIAM;ROONEY LEEANN;SHISHIDO HIDEKI;YANG ZHONGYING;OREGON HEALTH & SCIENCE UNIVERSITY |
发明人 |
SKACH WILLIAM;ROONEY LEEANN;SHISHIDO HIDEKI;YANG ZHONGYING |
分类号 |
G01N33/68 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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