| 摘要 |
<p>The invention relates to a process for preparing a bacterial product with antifungal activity of the Bacillus amylolichefaciens culture, by developing the inoculum by cultivating the microbial strain on a glucose- and mineral salts-containing medium, for 24...36 hours, at a temperature of 28...32°C, while stirring at 160...200 rpm, obtaining the bioprocess culture by cultivating the microbial strain on a glucose- and mineral salts-containing medium, for 72...120 hours, at 28...32°C, while stirring at 160...200 rpm, separating the bacterial biomass by centrifugation at 4000 rpm and a temperature of 4°C, adjusting the supernatant pH to 2.5...3.5, extracting it twice with ethyl acetate at a supernatant : solvent volume ratio of 1 : 2.5, evaporating the solvent at 40°C for 20...30 min, solubilizing the residue in distilled water and conditioning the microbial metabolites as emulsion, by encapsulation in natural polymer particles.</p> |
