TWO-PHOTON PROBE FOR MEASUREMENT OF PH IN HUMAN TISSUES

摘要

PURPOSE: A two-photon probe is provided to effectively measure the pH concentration of a gastric tissue, an esophagus tissue, and viable cells in a depth of 90-180 micrometers by a ratiometric method using a two-photon microscopy. CONSTITUTION: A two-photon probe of chemical formula 1 is able to measure a pH concentration in vivo. A method for preparing the two-photon probe comprises the steps of reacting a compound of chemical formula 2 with a mixture of isonicotinic acid and phosphorus oxychloride and preparing the compound of chemical formula 1. The compound of chemical formula 2 is prepared by reacting a mixture containing a compound of chemical formula 3 and hexamethylenetetramine in an organic solvent. The compound of chemical formula 3 is prepared by refluxing a mixture containing a compound of chemical formula 4 with CuBr2 in an organic solvent.