REACTION DEVICE, REACTION METHOD AND METHOD OF SYNTHESIZING cDNA

摘要

The present invention provides a device and a method whereby plural kinds of reaction operations and washing operations can be conducted in parallel without washing or replacing an instrument used in transferring a solution and the like in each operation. A reaction device having a plurality of projecting barriers provided in a line on one surface of a substrate, wherein the projecting barrier has at least one cutoff portion and an inner space capable of holding a droplet, and at least the face holding the droplets of the substrate surface has a contact angle to pure water of from 90 to 150 degrees. A reaction method using the above reaction device wherein a substance immobilized on magnetic beads is sequentially transferred in and between droplets of a solution containing a surface tension reducing agent that are held in the spaces for holding a droplet defined by the above-described projecting barriers by means of a magnet heated on the opposite surface of the substrate to the surface having the projecting barriers to thereby conduct reactions and washings.