NOVEL LIPASE FROM STREPTOMYCES SP. CS326 AND PROCESS FOR PREPARING THE SAME

摘要

PURPOSE: A lipase produced by Streptomyces sp. is provided to catalyze transesterification for producing biodiesel and to properly produce biodiesel. CONSTITUTION: A lipase for producing biodiesel is isolated from Streptomyces sp. (CS326) and is identified to have a single protein band through SDS-PAGE. The molecular weight of the lipase is 17 kDa. The optimal reaction temperature and pH of the lipase are 40 deg. C and pH 7.0. The lipase is a heat resistant enzyme. The lipid decomposition activity is increased to 100% by adding Triton X-100 and is suppressed to 5% by adding SDS. The lipid decomposition activity is completely suppressed in deoxycholic acid and is suppressed by 50% using EDTA and EGTA. The lipid decomposition activity is suppressed by Mg^2+, Fe^2+, Na^+, Mn^2+, Ca^2+, and Co^2+. The rate constant is Km=0.24mM and V_max=4.6mM/min/mg using Lineweaver-Burk plot.