DETECTION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
摘要
The present methods pertain to amplifying and/or detecting Staphylococcus aureus ("SA") and methicillin-resistant Staphylococcus aureus ("MRSA") nucleic acids based on a combined detection of Idhl as a SA marker and mecA as a MRSA marker. In certain embodiments the methods also pertain to amplifying and/or detecting one or more SCCmec integration sites or bridge regions. Primers and probes are suitable to be used in the present methods to detect SA and MRSA simultaneously in a single reaction or in separate reactions. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer ("FRET"), radiolabels, enzyme labels, and the like.
申请公布号
WO2013048583(A2)
申请公布日期
2013.04.04
申请号
WO2012US39275
申请日期
2012.05.24
申请人
ELITECH HOLDING B.V.;AFONINA, IRINA, A.;BELOUSOV, YEVGENIY, S.;MAHONEY, WALT
发明人
AFONINA, IRINA, A.;BELOUSOV, YEVGENIY, S.;MAHONEY, WALT