METHOD FOR AMPLIFYING AND SEQUENCING NUCLEIC ACID

摘要

PROBLEM TO BE SOLVED: To provide an apparatus and method for performing rapid DNA sequencing, such as genomic sequencing.

SOLUTION: This method for sequencing nucleic acids includes: a step of fragmenting large template nucleic acid molecules to generate a plurality of fragmented nucleic acids; a step of delivering the fragmented nucleic acids into aqueous microreactors in a water-in-oil emulsion; a step of amplifying the fragmented nucleic acids in the microreactors to form amplified copies of the nucleic acids and binding the amplified copies to beads in the microreactors; a step of delivering the beads to areas on a planar surface to produce an array of at least 10,000 reaction chambers wherein the plurality of reaction chambers include a single bead; and a step of performing a sequencing reaction simultaneously on the plurality of reaction chambers. The apparatus for sequencing nucleic acids is also disclosed.

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