METHOD FOR PREDICTION OF RISK OF DEVELOPING EARLY OCCUPATIONAL ALLERGIC DERMATOSES

摘要

FIELD: medicine. ^ SUBSTANCE: invention refers to medicine, and concerns a method for prediction of a risk of developing early occupational allergic dermatoses. The method involves venous blood sampling, genetic material recovery to be examined for polymorphy version A4889G of cytochrome P-450 1A1 by polymerase chain reaction followed by real-time high throughput sequencing. Conducting PCR requires specific primers C1A1-F Biotin - ggT gTT Aag TgA gAA ggT gAT T and C1A1-R Cag gAT AgC Cag gAA gAg AAA to be used; while high throughput sequencing involves specific primer C1A1-S ACC TCC Cag Cgg gCA A. If the derived pyrogram in position 4889 shows no adenine for guanine substitution and body exposure to an occupational hazard, the risk of developing early occupational allergic dermatoses less than 40% is predicted. If observing heterozygous version Cyp1A1*2C caused by adenine for guanine substitution in a nucleotide sequence in position 4889, and body exposure to said occupational hazard, the risk of 40%80% of developing early occupational allergic dermatoses is predicted. ^ EFFECT: method provides higher reproducibility and rate of the prediction analysis of the risk of occupational allergic dermatoses in case of body exposure to irritant and sensitising hazards. ^ 5 dwg, 2 ex