| 摘要 |
The disclosure provides a family of microscopes with components or discrete compact devices for illuminating an object with radiation of various spectral ranges suitable for multichannel fluorescence microscopy, so that an object is sequentially illuminated with different excitation spectra of particular fluorescent dyes. According to certain embodiments: several independent light-radiating surfaces are configured to radiate at different spectral ranges along an optical axis perpendicular to a plane defined by the surfaces and are spaced apart from each other, an optical illuminating system consisting of optical components, through which an illuminating ray path is directed at the object, wherein the surfaces are laterally offset from the optical axis of the optical illuminating system, and a deflecting mechanism configured for the sequential application of light coming from the light-radiating surfaces, so that as the direction changes, light enters the optical illuminating system and any lateral offset of the light-radiating surfaces is compensated. |
