| 摘要 |
The present invention relates to the use in a diagnostic hybridization assay of a molecular beacon probe for lowering: the effect of sequence variations in a nucleic acid analyte, and/or the IBL effect due to the possible opening of the stem-loop structure of a molecular beacon by way of (contaminants in the amplification) enzymes, which assay comprises the steps of contacting a set of primers and a sample containing the nucleic acid analyte to amplify the analyte and detecting the amplified analyte or its complement using a molecular beacon probe, wherein the molecular beacon probe comprises one or more nucleotides and/or nucleotide analogues that have an affinity increasing modification. The invention also relates to such molecular beacon probe and to a kit for performing a diagnostic assay using such molecular beacon probe. |
