METHOD FOR THE SELECTION OF RECOMBINANT CLONES COMPRISING A SEQUENCE ENCODING AN ANTIDOTE PROTEIN TO TOXIC MOLECULE

摘要

The present invention is related to a method for the selection of recombinant clones having integrated a gene of interest (6) and a nucleotide sequence (3) encoding a functional antidote protein (4) to a toxic molecule (5), wherein said recombinant clones are the ones which survive following their integration into a host cell (20) comprising in its genome a nucleotide sequence (21) encoding said toxic molecule (5).The present invention is also related to a nucleic acid construct, a vector comprising said nucleic acid construct, a host cell and a cloning and/or sequencing kit for performing said method.