Method of detecting an amount of a cholinesterase inhibitor in a sample

摘要

A method of detecting an amount of a cholinesterase inhibitor in a sample comprising steps of: (a) contacting the sample with an agent capable of recovering the cholinesterase inhibitor from the sample so that the cholinesterase inhibitor is recovered, wherein the agent comprises a reactivity towards phosphyl moieties; (b) isolating the recovered cholinesterase inhibitor from the sample; (c) contacting the isolated cholinesterase inhibitor from step (b) with a test cholinesterase wherein the cholinesterase activity of the test cholinesterase before step (a) is known; and (d) measuring the cholinesterase activity to determine the amount of cholinesterase inhibitor in the sample based on the inhibition of the test cholinesterase activity from the known activity of the test cholinesterase before step (a).