| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide new nucleic acid expression systems, expression constructs, methods for generating them, and methods of utilizing them to make biologically active nucleic acids, and, if desired, polypeptides. <P>SOLUTION: A method and constructs for expressing heterologous sequences of interest in eukaryotic cells using multiple-compartment expression systems. The systems, which may be comprised of a single construct or multiple constructs, utilize at least two different promoters which are each active within a different subcellular compartment of the same eukaryotic cell. The constructs are particularly useful for achieving enhanced in vivo expression of RNA molecules capable of modulating the expression of target genes. <P>COPYRIGHT: (C)2012,JPO&INPIT |
