XYLITOL PRODUCING MICROORGANISM INTRODUCED WITH ARABINOSE METABOLIC PATHWAY AND PRODUCTION METHOD OF XYLITOL USING THE SAME

摘要

PURPOSE: An arabinose metabolic pathway-introduced xylitol-producing strain and a method for producing xylitol using the same are provided to suppress arabitol generation. CONSTITUTION: A xylitol-producing strain(deposit number KCTC 11761BP) with suppressed arabitol generation is prepared by introducing a gene expression cassette containing an arabinoser metabolic pathway-associated gene into Candida sp. A method for procuring the strain comprises: a step of preparing a gene expression cassette having a terminator, a promoter, and a polynucleotide encoding L-arabinose isomerase; a step of preparing a gene having a promoter, a terminator, and a polynucleotide encoding L-ribrokinase; and a step of preparing a gene expression cassette having a promoter, a terminator, and a polynucleotide encoding L-ribrose-5-phosphate 4-epimerase.