A METHOD OF PRODUCING BIODIESEL USING CO-IMMOBILIZED LIPASES

摘要

PURPOSE: A biodiesel manufacturing method using concurrent immobilization lipase is provided to optimize maintenance of jathropa up to biodiesel production process and manufacture biodiesel from inedible fat. CONSTITUTION: A biodiesel manufacturing method comprises the following steps: using a concurrent immobilized lipase which a non-regioselective lipase and a 1,3-regioselective lipase are concurrently fixed in one carrier; and processing with reaction temperature at 36-54deg.C, agitation speed at 160-340rpm, 1-20 weight% of water content in reactive substrate, and 10-50weight% of concurrent lipase concentration based on weight% of the fat. The non-regioselective lipase is originated from Staphylococcus aureus, Penicillium cyclopium, Corynebacterium acnes, or Candida rugosa and the 1,3- regioselective lipase is originated from Rhizopus, Aspergillus, Mucor genus origin, pancreatic lipase, or rice bran. Ratio between the non-regioselective lipase in the concurrent immobilization lipase and the 1,3- regioselective lipase is 1:3-3:1 based on weight reference.