POTASSIUM CHANNEL MUTANTS OF THE YEAST SACCHAROMYCES CEREVISIAE AND THEIR USE FOR SCREENING EUKARYOTIC POTASSIUM CHANNELS

摘要

PROBLEM TO BE SOLVED: To provide processes for identifying inhibitors and activators of eukaryotic potassium channels.

SOLUTION: The process for identifying inhibitors of a eukaryotic potassium channel, in which (a) a mutated S. cerevisiae cell is used which does not express the three endogenous potassium channels TRK1, TRK2 and TOK1; (b) a eukaryotic potassium channel is expressed heterologously in this mutated S. cerevisiae cell; (c) the mutated S. cerevisiae cell is incubated together with a substance to be tested; and (d) the effect of the substance to be tested on the eukaryotic potassium channel is determined. Other subject matters are mutated S. cerevisiae cells which do not express TRK1, TRK2 and TOK1, and the preparation and use of these mutated S. cerevisiae cells.

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