发明名称 |
COMPLEX AND METHOD FOR ENHANCING NUCLEAR DELIVERY |
摘要 |
The use of at least one nucleic acid based nuclear localization signal including a natural or synthetic m3G-CAP is shown to increase transmembrane transport of a molecular cargo, in particular large molecules, into the nucleus. The use of natural and synthetic m3G-CAP is disclosed and the effect shown with a natural RNA 5′ end nuclear localization signal composed of a 2,2,7-trimethylguanosine CAP (m3G-CAP) coupled to fluorescent Streptavidin in one example, and an antisense oligonucleotide in another. A methylenephosphonate modified m3G-CAP is shown to have improved stability in human serum and in cytosolic extract.
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申请公布号 |
US2011306138(A1) |
申请公布日期 |
2011.12.15 |
申请号 |
US200913140070 |
申请日期 |
2009.12.17 |
申请人 |
SMITH EDVARD;MORENO PEDRO;STROEMBERG ROGER;WENSKA MALGORZATA |
发明人 |
SMITH EDVARD;MORENO PEDRO;STROEMBERG ROGER;WENSKA MALGORZATA |
分类号 |
C12N15/85;C07H19/20;C07K9/00;C07K14/00 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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