发明名称 |
Cell culture support and manufacture thereof |
摘要 |
<p>An object of the present invention is to provide a cell culture support making the detachment of a cell sheet easy as well as enabling the formation of a uniform cell sheet. The present invention relates to a method for manufacturing a cell culture support having a temperature responsive polymer immobilized onto the surface thereof via covalent bonding, the method including a coating step in which a composition including a monomer that can form the polymer by polymerization by radiation irradiation, an organic solvent and, in some cases, a prepolymer formed by polymerization of the monomer is coated onto the substrate having a surface containing a material which can be covalently bonded to the temperature responsive polymer by radiation irradiation to form a film on the surface of the substrate, a radiation irradiation step in which a polymerization reaction and a binding reaction between the substrate surface and the temperature responsive polymer are allowed to proceed by irradiating radiation to the film, and a drying step to dry the film.</p> |
申请公布号 |
EP2135940(B1) |
申请公布日期 |
2011.11.30 |
申请号 |
EP20090010850 |
申请日期 |
2008.03.12 |
申请人 |
DAI NIPPON PRINTING CO., LTD.;TOKYO WOMEN'S MEDICAL UNIVERSITY;CELLSEED INC |
发明人 |
WATANABE, MASANAO;HAGIWARA, KENICHI;OKANO, TERUO;YAMATO MASAYUKI;SHIMIZU TATSUYA;AKIYAMA YOSHIKATSU;WATANBE HIROYA;ASHIBA KEISUKE |
分类号 |
C12N5/00 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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