发明名称 |
6-O sulfated polysaccharides and methods of preparation thereof |
摘要 |
Disclosed are methods of 6-O-sulfating glucosaminyl N-acetylglucosamine residues (GlcNAc) in a polysaccharide preparation and methods of converting anticoagulant-inactive heparan sulfate to anticoagulant-active heparan sulfate and substantially pure polysaccharide preparations may by such methods. Also disclosed is a mutant CHO cell which hyper-produces anticoagulant-active heparan sulfate. Methods for elucidating the sequence of activity of enzymes in a biosynthetic pathway are provided. |
申请公布号 |
US8067196(B2) |
申请公布日期 |
2011.11.29 |
申请号 |
US20040473180 |
申请日期 |
2004.03.25 |
申请人 |
ROSENBERG ROBERT D.;ZHANG LIJUAN;BEELER DAVID L.;MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
发明人 |
ROSENBERG ROBERT D.;ZHANG LIJUAN;BEELER DAVID L. |
分类号 |
C12N15/09;C12P11/00;A61K8/73;A61Q5/12;A61Q19/00;C08B37/00;C12N5/10;C12N9/10;C12P19/04;C12P19/26;C12Q1/02;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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