发明名称 |
IMPROVED TECHNIQUES FOR TRANSFECTING PROTOPLASTS |
摘要 |
The invention relates to a method for the introduction of one or more molecules of interest in a plant cell protoplast by providing plant cell protoplasts, performing a first transfection of the plant cell protoplast with a composition that is capable of altering the regulation of one or more pathways selected from the group consisting of Mismatch Repair System and Non-Homologous End Joining and/or a composition that is capable of introducing DSBs, performing a second transfection of the plant cell protoplast with one or more molecules of interest such as mutagenic oligonucleotides and allowing the cell wall to form.
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申请公布号 |
CA2783551(A1) |
申请公布日期 |
2011.06.30 |
申请号 |
CA20102783551 |
申请日期 |
2010.12.20 |
申请人 |
KEYGENE N.V. |
发明人 |
BUNDOCK, PAUL;FIERENS-ONSTENK, BERNARDA GERHARD JOHANNA;LHUISSIER, FRANCK |
分类号 |
C12N15/82;A01H5/00;C12N15/10 |
主分类号 |
C12N15/82 |
代理机构 |
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地址 |
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