发明名称 |
Optimized DNA sequences encoding recombinant human bone morphogenetic protein-2 (rhBMP-2), preparation method and the uses thereof |
摘要 |
The invention discloses an optimized DNA sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) based on the Escherichia coli expression system and a method for the preparation of the rhBMP-2. Specifically, the invention provides the optimal DNA sequences suitable for Escherichia coli expression system, the methods for efficient preparation of the rhBMP-2, and the related construction of the recombinant bacteria, the expression and purification technologies. Compared with the traditional hBMP-2 gene without optimizing, the rhBMP-2 expression level of the optimized gene in Escherichia coli is increased by 50%. Additionally, this invention also provides a method for preparation long chain rhBMP-2 with enhanced renaturation efficiency and yield of purification.
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申请公布号 |
US7947821(B2) |
申请公布日期 |
2011.05.24 |
申请号 |
US20090623527 |
申请日期 |
2009.11.23 |
申请人 |
SHANGHAI REBONE BIOMATERIALS CO., LTD. |
发明人 |
LIU CHANGSHEN;LIN JUN;QIAN JIANGCHAO;YUAN YUAN |
分类号 |
C07H21/02;C07H21/04;C12N1/20;C12N15/63;C12N15/85;C12N15/86 |
主分类号 |
C07H21/02 |
代理机构 |
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地址 |
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