发明名称 |
MUTANT YQHD ENZYME FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION |
摘要 |
The present invention concerns a method for the production of a biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol comprising culturing a microorganism modified for an improved production of the biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a YqhD enzyme which catalytic efficiency toward NADPH is increased. The present invention also relates to a mutant YqhD enzyme comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the YqhD activity of the parent enzyme and the catalytic efficiency toward NADPH of the mutant YqhD is increased as compared with the catalytic efficiency toward NADPH of the parent enzyme. |
申请公布号 |
WO2011012697(A3) |
申请公布日期 |
2011.05.19 |
申请号 |
WO2010EP61106 |
申请日期 |
2010.07.30 |
申请人 |
METABOLIC EXPLORER;VOELKER, FRANCOIS;DUMON-SEIGNOVERT, LAURENCE;SOUCAILLE, PHILIPPE |
发明人 |
VOELKER, FRANCOIS;DUMON-SEIGNOVERT, LAURENCE;SOUCAILLE, PHILIPPE |
分类号 |
C12N9/02;C12P7/18;C12P7/26 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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