| 摘要 |
A fluorescence detector is provided to improve the non-linearity of the relationship between concentration and fluorescence intensity when the specimen concentration is high and thereby improve the dynamic range of the measurements. If it is known that the concentration of the specimen to be measured is high, a light beam restriction unit 6 whose aperture 6b in the passage direction of the excitation light Lex is short is used so that only the fluorescence being emitted from a region close to the incident end of the excitation light is condensed by the condensing lens 7 and led to the fluorescence side spectrometer 4 and detected. In this case, since the fluorescence that is emitted from a region after the passage of the excitation light through specimen solution S and its strong absorption by specimen solution S is not reflected in the measurement result, the linearity of the relationship between concentration and fluorescence intensity is improved despite the reduction in the fluorescence quantity, thus improving the quantification accuracy at high concentrations. If the specimen concentration is low, light beam restriction unit 6 with aperture 6a whose length is long in the passage direction of the excitation light Lex is used to improve sensitivity.
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