摘要 |
A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action. |
申请人 |
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY;BIONEER CORPORATION |
发明人 |
HOE, KWANG LAE;KIM, DONG UK;WON, MI SUN;YOO, HYANG SOOK;KIM, DONG SUP;PARK, HAN OH;CHUNG, KYUNG SOOK;JANG, YOUNG JOO;NAM, MI YOUNG;HAN, SANG JO;CHOI, SHIN JUNG;BAEK, SEUNG TAE;KIM, HYONG BAI;HEO, KYUNG SUN;LEE, HYE MI;LEE, MIN HO;PARK, JO YOUNG |