CARTILAGE PARTICLE TISSUE MIXTURES OPTIONALLY COMBINED WITH A CANCELLOUS CONSTRUCT

摘要

Mixtures, such as gels or pastes, comprising freeze-milled cartilage particles and exogenous growth factors are used for repairing chondral defects. Such mixtures may be applied to constructs comprising cancellous bone for implantation at the defect site. Suitable growth factors include variants of FGF-2, particularly variants that include a sole amino acid substitution for asparagine at amino acid 111 of the ß8-ß9 loop of the FGF-2 peptide. Such FGF-2 variants are released slowly and continuously at a constant rate from cartilage pastes. In other embodiments, the amino acid substituted for asparigine is glycine. Other variants that may be used include FGF-9 variants having truncated chains and a sole amino acid substitution in the ß8-ß9 loop of the FGF-9 peptide either for tryptophan at amino acid 144 or for asparagine at amino acid 143.