发明名称 |
DETECTION OF BACTERIA AND FUNGI |
摘要 |
<p>A method of detecting a ligase expressing micro-organism in a sample comprises steps of treating the sample under conditions that inhibit the activity of ATP-dependent ligase from mammalian cells but which do not inhibit the activity of the microbial ligases, contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for ligase activity in the sample, incubating the thus contacted sample under conditions suitable for ligase activity; and specifically determining the presence and/or the amount of a ligated nucleic acid molecule resulting from the action of the ligase on the substrate nucleic acid molecule to indicate the presence of the ligase expressing micro-organism. The micro-organism may be a fungus or a bacterium or both. High pH conditions may be employed to inactivate mammalian ligases. Related kits are described.</p> |
申请公布号 |
WO2010119270(A1) |
申请公布日期 |
2010.10.21 |
申请号 |
WO2010GB00781 |
申请日期 |
2010.04.19 |
申请人 |
MOMENTUM BIOSCIENCE LTD;WILSON, STUART;MULLEN, WILLIAM |
发明人 |
WILSON, STUART;MULLEN, WILLIAM |
分类号 |
C12Q1/04 |
主分类号 |
C12Q1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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