摘要 |
The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20 % of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ß- glucanase and endogenous cellobiohydrolase is less than 20 % of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75 %, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided. |
申请人 |
DYADIC NEDERLAND B.V.;PUNT, PETER, J.;BURLINGAME, RICHARD, PAUL;PYNNONEN, CHRISTINE, M.;VERDOES, JACOBA;OLSON, PHILLIP,T.;WERY, JAN;VISSER, JOHANNES, HEINRICH;EMALFARB, MARK, A.;VISSER, JACOB |
发明人 |
PUNT, PETER, J.;BURLINGAME, RICHARD, PAUL;PYNNONEN, CHRISTINE, M.;OLSON, PHILLIP,T.;WERY, JAN;VISSER, JOHANNES, HEINRICH;EMALFARB, MARK, A.;VISSER, JACOB;VERDOES, JAN, CORMELIS |