METHOD OF OBTAINING POPULATION OF HUMAN CD4+CD25+Foxp3+ T-LYMPHOCYTES ex vivo, DISEASE TREATMENT METHOD

摘要

FIELD: chemistry; biochemistry. ^ SUBSTANCE: invention relates to biotechnology, in particular to obtaining cell populations of T-lymphocytes, and can be used in medicine. The method involves isolation a population of CD4+T-cells from peripheral blood of a patient and culturing the cells in a growth medium containing monoclonal antibodies to CD28 (anti-CD28) in amount of 0.5-10 mcg/ml and to CD3 (anti-CD3) in amount of 0.5-10 mcg/ml, TGF-beta 1 (1-50 ng per ml) and interleukin-2 (IL-2) in amount of 10-1000 units/ml. The obtained population of CD4+T-cells is used in a method of treating diseases characterised by pronounced reduction of amount and/or functional activity of regulatory T-cells. ^ EFFECT: invention enables obtaining of a population of lymphocytes rich in regulatory T-cells ex vivo. ^ 2 cl, 8 dwg