| 摘要 |
PROBLEM TO BE SOLVED: To provide methods of high throughput and quantitative analysis of glycoproteins and glycoprotein profiling. SOLUTION: The invention provides a method for identifying and quantifying polyglycopeptides in a sample. The method includes the steps of: immobilizing glycopolypeptides to a solid support; cleaving the immobilized glycopolypeptides, thereby releasing non-glycosylated peptides and retaining immobilized glycopeptides; releasing the glycopeptides from the solid support; and analyzing the released glycopeptides. The method further includes the step of identifying one or more glycopeptides, for example, using mass spectrometry. COPYRIGHT: (C)2010,JPO&INPIT |
