| 摘要 |
A method and a test kit for rapid detection of specific nucleic acid sequences, especially for detection of mutations or single nucleotide polymorphisms (SNPs), in which the detection reaction takes place in two steps. The first step involves the target-specific amplification reaction, coupled with the probe-hybridization reaction using fluorescence-labeled allele-specific amplification primers. In the second step, the fluorescence is detected by means of commercial fluorescence readers. Genotyping is carried out from the ratio of the end-point fluorescence of the samples and negative controls.
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