METHOD FOR SCREENING ANTI-CANCER AGENTS INHIBITING INTERACTION OF CYCLOOXYGENASE-2 AND P53

摘要

PURPOSE: A method for screening an anti-cancer drug is provided to screen antagonism to tumorigenesis and metastasis based on the molecular mechanism of p53 suppression. CONSTITUTION: A method for screening an anticancer drug which suppresses the interaction of cyclooxygenase-2(COX-2) and p53 comprises: a step of contacting a COX-2 containing p53-binding domain(amino acid 1-126) or a fragment thereof with p53 having a DNA-binding domain under the presence of a test drug; and a step of determining the suppression of interaction between COX-2 and p53 by comparing with a control group in which a test drug is not contained. The interaction between the COX-2 and p53 occurs between the p53-binding domain of COX-2 and the DNA-binding domain of p53. The binding site of COX-2 and p53 is an amino acid of sequence number 2. The interaction of the COX-2 and p53 is measured through a GST pull down assay, immunoprecipitation assay, and EMSA(electrophoretic mobility shift assay).