USE FOR CD45+ CELLS OR A CD45+ CELL CULTURE FLUID FOR PROMOTING THE GROWTH OF MESENCHYMAL STEM CELLS

摘要

The present invention relates to a use for CD45+ cells or a CD45+ cell culture fluid for promoting the growth of mesenchymal stem cells; a composition for promoting the growth of mesenchymal stem cells comprising CD45+ cells or a CD45+ cell culture fluid; and a culturing method for promoting the growth of mesenchymal stem cells by adding CD45+ cells or a CD45+ cell culture fluid to mesenchymal stem cells and allowing culturing to proceed. If culturing is carried out by adding CD45+ cells or a CD45+ cell culture fluid to mesenchymal stem cells, then the colony-forming ability of the mesenchymal stem cells is increased by at least three times as compared with culturing of mesenchymal stem cells alone. Moreover, CD29+ cells which have been cultured with treatment by CD45+ cells or a CD45+ cell culture fluid maintain, unchanged, the morphological characteristics of mesenchymal stem cells, cell markers, and inherent characteristics such as pluripotency. Thus CD45+ cells or a CD45+ cell culture fluid can be used to promote the growth of mesenchymal stem cells, and a culturing method in which CD45+ cells or a CD45+ cell culture fluid is added to mesenchymal stem cells and culturing is allowed to proceed can be used to grow mesenchymal stem cells in sufficient number to allow them to be used in cell therapy.