发明名称 |
IMMUNOASSAY ON A PREBLOCKED SOLID SURFACE |
摘要 |
A method far immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate o a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.
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申请公布号 |
CA2000685(A1) |
申请公布日期 |
1990.05.17 |
申请号 |
CA19892000685 |
申请日期 |
1989.10.13 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
HENDERSON, GLENN L.;HOKE, RANDAL A.;HOPKINS, ANNE C.;MCLAURIN, DANIEL A. |
分类号 |
G01N33/543;G01N33/569;G01N33/58 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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