The invention provides a method for determining a degree of phosphorylation of a substrate, for example a peptide substrate, using a fluorescence probe that acts alone or with another material and has a lifetime that varies when in proximity to a phosphate, the method comprising: causing the fluorescence probe to fluoresce; measuring a time response of the fluorescence, and analysing the fluorescence time response to identify a fluorescence component having a lifetime associated with phosphorylated substrate and a fluorescence component having a lifetime associated with un-phosphorylated substrate.
申请公布号
WO2009001051(A2)
申请公布日期
2008.12.31
申请号
WO2008GB02134
申请日期
2008.06.20
申请人
EDINBURGH INSTRUMENTS LIMITED;THE UNIVERSITY COURT OF THE UNIVERSITY OF DUNDEE;GRAY, ALEXANDER;PATERSON, MICHAEL, JOHN;GAKAMSKY, DMITRY