IMPROVED VARIANTS OF THE BACILLUS LICHENIFORMIS ALPHA-AMYLASE

摘要

Variants of B. licheniformis alpha-amylase advantageously exhibit improve d enzymatic performance. Suitable variants include those with an altered cha rge distribution on the surface of the enzyme or with altered active site re sidues. Structural modeling can inform the choice of amino acid modification s so that modified amino acids correspond to residues found in more active a lpha amylases, for example. Compositions comprising the variants are useful in methods of cleaning surfaces, laundering textiles, desizing, treating sta rch, e.g., liquefaction and saccharification, and hydrolyzing biofilms off v arious substrates.