发明名称 |
Quantitative Two-Photon Flow Cytometry |
摘要 |
A method and apparatus of multi-dye analysis of particles using flow cytometer. The method includes dying particles to be detected using two or more dyes; urging the particles through a capillary in a non-uniform flow; exciting a first of the particles within the capillary using a multiphoton excitation laser beam causing the two or more dyes each to fluoresce thereby producing a first output signal and a second output signal respectively; and detecting the first output signal and the second output signal. A second of the particles within the capillary being excited using the multiphoton excitation laser beam causing the two or more dyes each to fluoresce thereby producing a third output signal and a forth output signal respectively. The method finally includes comparing a ratio of the first output signal and the second output signal to a ratio of the third output signal and the forth output signal to detect a desired change in the particles.
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申请公布号 |
US2008292555(A1) |
申请公布日期 |
2008.11.27 |
申请号 |
US20050662835 |
申请日期 |
2005.09.16 |
申请人 |
YE JING YONG;NORRIS THEODORE B;ZHONG CHENG F;BAKER JAMES R |
发明人 |
YE JING YONG;NORRIS THEODORE B.;ZHONG CHENG F.;BAKER JAMES R. |
分类号 |
A61K49/00;A61B5/00;C12Q1/02 |
主分类号 |
A61K49/00 |
代理机构 |
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地址 |
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