| 摘要 |
A thermal cycling device ( 3 ) device a number of fixed thermal zones ( 11, 12, 13 ) and a fixed conduit ( 10 ) passing through the thermal zones. A controller maintains each thermal zone including its section of conduit ( 10 ) at a constant temperature. A series of droplets flows through the conduit ( 10 ) so that each droplet is thermally cycled, and a detection system detects fluorescence from droplets at all of the thermal cycles. The conduit is in a single plane, and so a number of thermal cycling devices may be arranged together to achieve parallelism. The flow conduit comprises a channel ( 17 ) and a capillary tube ( 10 ) inserted into the channel. The detection system may perform scans along a direction to detect radiation from a plurality of cycles in a pass.
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