CRYSTALS OF A BACTERIAL METABOLITE-SENSING RIBOZYME AND USE THEREOF FOR ANTIBIOTIC DISCOVERY

摘要

A crystalline GlmS ribozyme, optionally but in some embodiments preferably in dehydrated (including partially dehydrated) form is described, along with the crystal structure thereof. The invention provides a method of identifying a GlmS binding compound (or compound that facilitates or inhibits cleavage of GlmS), comprising: (a) providing in a computer a three-dimensional structural representation of GlmS or a glucosamine-6-phosphate (GlcN6P) and/or glucose-6-phosphate (Glc6P) binding portion thereof; and (b) screening in the computer a candidate compound for an ability to bind to said three-dimensional structural representation. In some embodiments the three-dimensional structural representation is provided at a resolution of from 4.0 or 3.0 Å, to 2.0 or 1.0 Å.