| 摘要 |
PROBLEM TO BE SOLVED: To quickly and highly accurately detect the single-nucleotide mutation, using simple operations. SOLUTION: A nucleic acid 4 containing a target base sequence is supplied to LCR reaction to amplify the target base sequence, and the obtained LCR reaction product 16 is subjected to LAMP reaction. An LCR primer set 10 in an LCR reaction solution 6 and an LAMP primer set 20 in an LAMP reaction solution 26 are preferably set to prescribed concentrations. The supply of the LCR reaction product 16 to the LAMP reaction enables to reduce the cycle number of the LCR reaction repeating heating and cooling, and a mutation can be detected with high accuracy using the LAMP reaction that does not need the heating and the cooling without depending on a real time measurement. Thus, even when a double-strand DNA is analyzed, the number of heating-cooling operations can be reduced, and the mutation can highly accurately be analyzed without depending on a real time measurement. COPYRIGHT: (C)2009,JPO&INPIT
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