| 摘要 |
A method for in vitro maturation of a porcine oocyte is provided to increase in vitro maturation rate of immature porcine oocyte, and reduce intrusion of multiple sperms, increase normal fertilization rate, and enhance blastocyst formation rate in the in vitro fertilization. A method for in vitro maturation of a porcine oocyte comprises the steps of: adding theca follicle into an in vitro maturation medium, which is prepared by adding 26.19 mM NaHCO3, 3.05 mM glucose, 0.91 mM sodium pyruvate, 75 mug/ml sodium penicillin G, 50 mug/ml streptomycin sulfate and 0.1% polyvinyl alcohol(PVA) in tissue culture medium(TCM) 199, in an amount of 1-6 theca follicle with the size of 2-4 mm per 2 ml of medium, and performing in vitro culture of the porcine oocyte in the in vitro maturation medium for 36-48 hours. |
