发明名称 |
FLUORESCENCE MICROSCOPE |
摘要 |
An object of the present invention is to obtain clear images when observing a specimen at different positions in the optical-axis direction of a detection optical system, using the sheet illumination. A fluorescence microscope of the present invention includes a sheet-illumination optical system that causes sheet-like excitation light along a plane of incidence to be incident on a specimen; an image-acquisition optical system that includes an objective lens having an optical axis intersecting the plane of incidence and that images fluorescence collected by the objective lens to obtain a fluorescence image; a driving part that moves the specimen in the optical-axis direction of the objective lens; and a positional-shift correction unit that, when a focus position of the image-acquisition optical system relative to an illumination position of the excitation light changes due to movement of the specimen in the optical-axis direction by the driving part, corrects a positional shift between the illumination position of the excitation light and the focus position of the image-acquisition optical system. |
申请公布号 |
US2016241758(A1) |
申请公布日期 |
2016.08.18 |
申请号 |
US201615041330 |
申请日期 |
2016.02.11 |
申请人 |
OLYMPUS CORPORATION |
发明人 |
DOHI Masahito;KIKUCHI Yu |
分类号 |
H04N5/225;G02B21/16;G02B21/36;H04N5/232 |
主分类号 |
H04N5/225 |
代理机构 |
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代理人 |
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主权项 |
1. A fluorescence microscope comprising:
a sheet-illumination optical system that causes sheet-like excitation light along a plane of incidence to be incident on a specimen; an image-acquisition optical system that includes an objective lens having an optical axis intersecting the plane of incidence and that images fluorescence collected by the objective lens to obtain a fluorescence image; a driving part that moves the specimen in the optical-axis direction of the objective lens; and a positional-shift correction unit that, when a focus position of the image-acquisition optical system relative to an illumination position of the excitation light changes due to movement of the specimen in the optical-axis direction by the driving part, corrects a positional shift between the illumination position of the excitation light and the focus position of the image-acquisition optical system. |
地址 |
Tokyo JP |