摘要 |
<p>The present invention relates to a biosensor for point-of-care testing (POCT) whose detection sensitivity was remarkably improved by introducing to membrane strip chromatographic assay system a successive cross-flow procedure for immune reaction and enzymatic reaction. The present invention relates to a membrane strip biosensor system that comprises (a) a membrane pad ( 10 ) for sample application, (b) a membrane pad ( 20 ) for release of detection binding component, wherein the membrane pad ( 20 ) contains label-linked binding component for detection in a dry state, (c) a signal generation membrane pad ( 30 ) with immobilized binding component for capture, (d) a membrane pad ( 40 ) for absorption of vertical flow medium, (e) a membrane pad ( 50 ) for the supply of substrate solution for enzyme, (f) a membrane pad ( 60 ) for absorption of horizontal flow medium and (g) substrate solution, wherein the system has a cross-arrangement of two groups of the membrane pads, (I) one group of vertically arranged pads, wherein the pad ( 10 ) is partially superimposed and fixed in length at the end of the pad ( 20 ), and the pad ( 20 ) and the pad ( 40 ) are partially superimposed and fixed in length at the both ends of the signal generating membrane pad ( 30 ), respectively; and (II) the other group of horizontally arranged pads, wherein the pad ( 50 ) and pad ( 60 ) are, at the time of signal generation, partially superimposed and fixed at the both lateral sides of the signal generation membrane pad ( 30 ), respectively.</p> |