TETANUS TOXIN FRAGMENT C BASED IMAGING AGENTS AND METHODS, AND CONFOCAL MICROSCOPY DATASET PROCESSES

摘要

Methods for purifying Tetanus Toxin Fragment C comprising obtaining a sup ernatant comprising soluble Tetanus Toxin Fragment C and purifying Tetanus T oxin Fragment C under native conditions to obtain a substantially purified T etanus Toxin Fragment C. Imaging agents comprising a Tetanus Toxin Fragment C and a reporter, and methods thereof. Methods comprising processing confoca l microscopy datasets to provide a 360 degree average fluorescence intensity profile from the center of a spheroid towards the outer edge of the spheroi d.